Plant Transcription Factor Database
Previous version: v3.0
Oryza rufipogon
HSF Family
Species TF ID Description
ORUFI01G22600.1HSF family protein
ORUFI01G26140.1HSF family protein
ORUFI01G33030.1HSF family protein
ORUFI01G34020.1HSF family protein
ORUFI02G09810.1HSF family protein
ORUFI02G18130.1HSF family protein
ORUFI02G19790.1HSF family protein
ORUFI03G04330.1HSF family protein
ORUFI03G09120.1HSF family protein
ORUFI03G19200.1HSF family protein
ORUFI03G35260.1HSF family protein
ORUFI03G35260.2HSF family protein
ORUFI03G35260.3HSF family protein
ORUFI03G38880.1HSF family protein
ORUFI03G43230.1HSF family protein
ORUFI04G23680.1HSF family protein
ORUFI05G25150.1HSF family protein
ORUFI06G19310.1HSF family protein
ORUFI06G19790.1HSF family protein
ORUFI07G04770.1HSF family protein
ORUFI07G25040.1HSF family protein
ORUFI08G19920.1HSF family protein
ORUFI08G25100.1HSF family protein
ORUFI09G13600.1HSF family protein
ORUFI09G13720.1HSF family protein
ORUFI09G18440.1HSF family protein
ORUFI10G10710.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645